Re: eosinophil staining on frozen sections
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From: | "J. A. Kiernan" <jkiernan@julian.uwo.ca> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
On Mon, 24 Jan 2000 CKlemenich@chob.edu wrote:
> Does anyone have a method for intensifing the staining of the eosinophils
> on a frozen section using the standard H & E ? Would adjusting the pH of
> the eosin help stain the eosinophils better ? The pathologist here have
> been asking this question for years and I do not have an answer.
Advise the pathologist to buy a textbook of histological
technique. He should know the answer to this question.
Raising the pH of the eosin will reduce the staining of
acidophilic objects other than eosinophils, which
consequently become easier to see. At pH 9 the
cytoplasmic granules of these cells and of Paneth cells
in the intestine (also sperm tails in testis) are the
only things stained by anionic dyes. At pH 8, which is less
likely to remove the sections from the slides, you will
also get some staining of red blood cells, but nothing
otherwise in cytoplasm and collagen.
John A. Kiernan,
Department of Anatomy & Cell Biology,
The University of Western Ontario,
LONDON, Canada N6A 5C1
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