Re: collagen
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From: | Amos Brooks <atbrooks@snet.net> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hi,
Try Collagen IV from DAKO (N1536 <-- I think). Use Proteinase K for 5
min. then primary for 30 min. Then LSAB2 for 10 min each.
Amos Brooks
Mark Elliott wrote:
> I have been asked to find out what collagen antibodies are available that
> work on formalin-fixed parafin-embedded tissues. We hope to do this
> using the DAKO autostainer. Any help would be greatly appreciated.
>
> Mark
>
> Mark Elliott, PhD
> Research Associate
> UBC-Pulmonary Research Lab
> St. Paul's Hospital
> Vancouver, BC Canada V6Z 1Y6
> 604-806-8351 (FAX)
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