Re: collagen

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From:Amos Brooks <> (by way of Marvin Hanna)
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    Try Collagen IV from DAKO (N1536 <-- I think). Use Proteinase K for 5
min. then primary for 30 min. Then LSAB2 for 10 min each.
Amos Brooks

Mark Elliott wrote:

> I have been asked to find out what collagen antibodies are available that
> work on formalin-fixed parafin-embedded tissues.  We hope to do this
> using the DAKO autostainer.  Any help would be greatly appreciated.
> Mark
> Mark Elliott, PhD
> Research Associate
> UBC-Pulmonary Research Lab
> St. Paul's Hospital
> Vancouver, BC Canada V6Z 1Y6
> 604-806-8351 (FAX)

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