Re: background in CD3s
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From: | "Nicola Falconer" <njfja@njfja.screaming.net> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Dear Sandosis
I have found the most common cause of background staining to be caused by
using the primary antibody at tooooo strng a concentration. Even prediluted
antibodies can often be diluted 1/2 to 1/10. Not only can this clean up your
slides, it also allows budgets to go a bit further (very important in the
cash strapped NHS in the UK).
Regards
John Auld
Dept of Immunocytochemistry
Royal Free Hospital
London
England
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