Re: background in CD3s

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From:"Nicola Falconer" <> (by way of Marvin Hanna)
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Dear Sandosis

I have found the most common cause of background staining to be caused by
using the primary antibody at tooooo strng a concentration. Even prediluted
antibodies can often be diluted 1/2 to 1/10. Not only can this clean up your
slides, it also allows budgets to go a bit further (very important in the
cash strapped NHS in the UK).


John Auld
Dept of Immunocytochemistry
Royal Free Hospital

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