Date sent: Mon, 15 Jan 2001 14:53:47 -0600
From: Jeneile Marlow <Jeneile-Marlow@mail.omrf.ouhsc.edu>
To: "'firstname.lastname@example.org'" <email@example.com>
> Hi, I am doing cryo sections on animal tissue and will be doing snap
> freezing with liquid nitrogen. The tissue specimens will be fixed before
> hand in 4% Para formaldehyde, I do not want to store in sucrose overnight
> because of the potential for interference with immunohistochemistry. Does
> anyone have any ideas on what I can use as substitute? Thanks Jenny.
I would tend to leave specimens in buffer (PBS) overnight after fix,
but also use sucrose. So I was interested in your comment on the
interference of sucrose with IHC, could you tell me what problems
you have encountered.
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