Date sent:      	Mon, 15 Jan 2001 14:53:47 -0600
From:           	Jeneile Marlow <Jeneile-Marlow@mail.omrf.ouhsc.edu>
Subject:        	Sucrose
To:             	"'histonet@pathology.swmed.edu'" <histonet@pathology.swmed.edu>

> Hi, I am doing cryo sections on animal tissue and will be doing snap
> freezing  with liquid nitrogen. The tissue specimens will be fixed before
> hand in 4% Para formaldehyde, I do not want to store in sucrose overnight
> because of the potential for interference with immunohistochemistry. Does
> anyone have any ideas on what I can use as substitute? Thanks Jenny.
> 
Jenny,
I would tend to leave specimens in buffer (PBS) overnight after fix, 
but also use sucrose. So I was interested in your comment on the 
interference of sucrose with IHC, could you tell me what problems 
you have encountered.
Terry.