Re: Sucrose

From:Huib Croes <>


I hope that the following mini-review gives an answer to your question.

CJF van Noorden et al
Polyvinyl alcohol and other tissue protectants in enzym histochemistry: a
consumer's guide
Histochemical Journal 21, 373-379 (1989)


Huib Croes
Cath Univ. Nijmegen
Dept. Cell Biology
PO BOX 9101
6500 HB Nijmegen
----- Original Message -----
From: "Tahir Mahmood" <tahir@MIT.EDU>
To: "Jeneile Marlow" <>;
Sent: dinsdag 16 januari 2001 4:33
Subject: Re: Sucrose

> i infuse with sucrose for 2 hours at room temp, and it has worked fine so
> i have also been told of an alternative procedure where the samples are
> infused with a sucrose/PVP mixture. Does anyone know why PVP is used? Is
> any better as a cryoprotectant? any possible problems?
> thanks a bunch,
> -Tahir
> At 02:53 PM 1/15/01 -0600, Jeneile Marlow wrote:
> >Hi, I am doing cryo sections on animal tissue and will be doing snap
> >freezing  with liquid nitrogen. The tissue specimens will be fixed before
> >hand in 4% Para formaldehyde, I do not want to store in sucrose overnight
> >because of the potential for interference with immunohistochemistry. Does
> >anyone have any ideas on what I can use as substitute? Thanks Jenny.
> >

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