Re: CD3 has too much background, ca you help?

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From:Jeffrey S Crews <cruzetti@juno.com> (by way of Marvin Hanna)
To:histonet@histosearch.com
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How long/ what temp do you do the primary for? What does your negative
control look like?                                    jc

On Thu, 27 Jan 2000 10:17:13 -0600 "Sandy Julsing"
<sandy.julsing@thedacare.org> writes:
>Histo-buddies
>     Our CD3 has too much background stain including in the gastric
>glands. Our protocol is...
>
>-DAKO pre-dilute CD3
>-antigen retrieval with citrate buffer pH 5.8-6.2 for 20 min with a 20
>min cool down
>-LSAB2 detection kit 10min/10min/5min
>-Stained on the DAKO autostainer
>
> Any help would be greatly appreciated, thanks,  Sandosis
>
>

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