Re: APES: rinsing - ?acetone and/or water

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From:"J. A. Kiernan" <> (by way of Marvin Hanna)
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On Mon, 24 Jan 2000, Rebecca S Smith wrote:

> It seems that extremely numerous rinses following coating is the BEST thing
> you can do for making "Perfect" APES coated slides!

  Are these lots of rinses in water? If so, do you rinse
  in acetone between the APES and the water, and if so,
  how much. As we've seen from all the messages in the
  last few days, some people go straight from APES
  solution into water, whereas others interpose one or
  more rinses in acetone.

  The traces of water present in acetone (I think it's
  about 0.5% on the label) are necessary for the
  combination of APES with glass. My experience, for
  what it's worth, is that if you don't rinse in
  acetone first, the slides are often rather cloudy.
  This doesn't seem to matter, because they're
  transparent again once the coverslips are in place,
  but it is a bit off-putting to mount sections on
  slides that don't look clean. Bought +vely
  charged slides aren't cloudy.

 John A. Kiernan,
 Department of Anatomy & Cell Biology,
 The University of Western Ontario,
 LONDON,  Canada  N6A 5C1

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