RE: oil immersion lens cleaning

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From:jim <jim@proscitech.com.au> (by way of Marvin Hanna)
To:histonet@histosearch.com
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You have my sympathy for working with grots Ian (been there myself), but we
are
talking about human nature, rather than the principle "do you have to
frequently clean to have the objective live."
Your department may be full of inverted scopes, but I would be surprized if
one
in a hundred scoped in this world was inverted.
Cheers
Jim Darley
ProSciTech



On Monday, January 24, 2000 11:04 PM, Ian Montgomery
[SMTP:ian.montgomery@bio.gla.ac.uk] wrote:
> >Date: Mon, 24 Jan 2000 14:21:40 +1000
> >From: jim <jim@proscitech.com.au>
> >Subject: FW: oil immersion lens cleaning
> >To: "histonet@pathology.swmed.edu" <histonet@pathology.swmed.edu>
> >
> >	Inverted optics are uncommon. If the recommended highly viscous oil is
> used,
> >there should be no problem with this running. Again, excess could be just
> wiped
> >off before application of another drop before the next use.
> >
>
> Jim,
> 	Histonet is not just confined to Pathology labs. Here in the
> Davidson/Wolfson/West Medical complex at Glasgow University, buildings that
> cover Physiology/PharmacologyBiochemistry and all the variations of these
> subjects the upright microscope is the rarity. Inverted microscopes are the
> norm. from routine use to experimental setups and confocal. Indeed, I'm
> sure if you go into any modern University department covering these
> subjects then you'll find inverted microscopes are the major microscope type.
>
> 	I agree that there shouldn't be a problem if users wiped the excess
>from
> the objective after use but human nature doesn't seem to work that way. I
> get blue in the face telling users they only need a very small drop but do
> they listen, nope, if a wee drop is good then a big drop must be even
> better. I use felt washers on the oil objectives of inverted microscopes
> and I can assure you they get saturated with oil on a regular basis.
> 'Wasn't me, must have been *****. I only use a small drop.'
> 	Immersion oil is bad enough but wait until you get the mixture of
>oil and
> the vaseline that was used to seal the slide plus the physioplogical salt
> solutions that bathe the specimens. After that lot immersion oil is a minor
> inconvenience.
> Ian.
>




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