RE: free-floating sections

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From:Tamara Howard <> (by way of Marvin Hanna)
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Just my opinion, but I think the best way to deal with these is in some
kind of well-plates, although this sort of depends on your sample size.
Either 96-well or some kind of microtiter plates - you can put each
section in its own little well and you don't use much reagent. Solution
changes can either be done by moving the section from well to well if your
sections are sturdy (on a smooth stick or glass rod) or you can carefully
pipet the old solution out and add the new one. Or, if you are very brave
(or foolhardy!) you could aspirate the solutions - a P200 tip on your
usual aspirator line - but this is usually disastrous at some
point...usually the final wash.

I've also stained floaties in microcentrifuge tubes, but this is easier if
it is easy to see the tissue. I use the well-plates for tiny and/or
difficult to see samples, and do the solution changes under a dissecting

Good luck!

Tamara Howard

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