Chris,
This has already been touched on recently. The clue is in the name
eosinoPHIL - lover of eosin.
Just let the section briefly meet the eosin, the eosinophils will suck the
stuff up and (virtually) nothing else will.

Tee

-----Original Message-----
From: p=NHS NATIONAL INT;a=NHS;c=GB;dda:RFC-822=CKlemenich(a)chob.edu;
Sent: 24 January 2000 17:24
To: p=NHS NATIONAL
INT;a=NHS;c=GB;dda:RFC-822=histonet(a)pathology.swmed.edu;
Subject: eosinophil staining on frozen sections


Does anyone have a method  for  intensifing the staining of the eosinophils
on a frozen section  using the standard  H & E ?  Would adjusting the pH of
the eosin help stain the eosinophils better ?  The pathologist here have
been asking this question for years and I do not have an answer.

Chris Klemenich
Kaleida Health - Childrens Hospital of Buffalo
ckemenich@kaleidahealth.org