RE: decal

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From:"Connolly, Brett" <brett_connolly@merck.com> (by way of Marvin Hanna)
To:histonet@histosearch.com
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Luann,
I have used the following:

16	g/L Trizma Base (Sigma)
100	g/L EDTA (Sigma)

dissolve and pH to 7.0 with NaOH.

Suspend bones in the solution with constant agitation (on a stir plate). It
takes a while, but is good if you want to do in situ hybridization or IHC.

Brett


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com

> ----------
> From: 	LuAnn Anderson[SMTP:ander093@gold.tc.umn.edu]
> Sent: 	Tuesday, January 25, 2000 1:01 PM
> To: 	histonet@pathology.swmed.edu
> Subject: 	decal
>
> Hi all,
> I am in need of the protocol for EDTA decalcification-percentage and pH of
>
> solution.  I have a pathologist who wants this method used and while I
> have
> found numerous references to EDTA(Sheehan, Carson, Bancroft etc) none give
> the
> solution details.  Thanks so much,  LuAnn
>
>
>




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