RE: decal
<< Previous Message | Next Message >>
From: | "Connolly, Brett" <brett_connolly@merck.com> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Luann,
I have used the following:
16 g/L Trizma Base (Sigma)
100 g/L EDTA (Sigma)
dissolve and pH to 7.0 with NaOH.
Suspend bones in the solution with constant agitation (on a stir plate). It
takes a while, but is good if you want to do in situ hybridization or IHC.
Brett
Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
WP26A-3000
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075
e-mail: brett_connolly@merck.com
> ----------
> From: LuAnn Anderson[SMTP:ander093@gold.tc.umn.edu]
> Sent: Tuesday, January 25, 2000 1:01 PM
> To: histonet@pathology.swmed.edu
> Subject: decal
>
> Hi all,
> I am in need of the protocol for EDTA decalcification-percentage and pH of
>
> solution. I have a pathologist who wants this method used and while I
> have
> found numerous references to EDTA(Sheehan, Carson, Bancroft etc) none give
> the
> solution details. Thanks so much, LuAnn
>
>
>
<< Previous Message | Next Message >>