RE: decal

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From:"Connolly, Brett" <> (by way of Marvin Hanna)
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I have used the following:

16	g/L Trizma Base (Sigma)
100	g/L EDTA (Sigma)

dissolve and pH to 7.0 with NaOH.

Suspend bones in the solution with constant agitation (on a stir plate). It
takes a while, but is good if you want to do in situ hybridization or IHC.


Brett M. Connolly, Ph.D.
Merck Research Laboratories
Department of Pharmacology
PO Box 4
West Point, PA 19486
Ph. 215-652-2501
FAX 215-652-2075

> ----------
> From: 	LuAnn Anderson[]
> Sent: 	Tuesday, January 25, 2000 1:01 PM
> To:
> Subject: 	decal
> Hi all,
> I am in need of the protocol for EDTA decalcification-percentage and pH of
> solution.  I have a pathologist who wants this method used and while I
> have
> found numerous references to EDTA(Sheehan, Carson, Bancroft etc) none give
> the
> solution details.  Thanks so much,  LuAnn

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