RE: Problems w/cutting monkey brains

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From:"McCollough, Carol" <> (by way of Marvin Hanna)
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I suspect your waterbath is too cool.  It needs to be set about 15 degrees
cooler than the melting point of your paraffin.  If you are using one of the
Paraplasts, then 45-47 degrees is good.  Hope this helps.

Regards -
Carol B. McCollough, HT(ASCP)
Diagnostics & Histology Laboratory Manager
Maryland Department of Natural Resources
Fisheries Service
Cooperative Oxford Laboratory
904 S. Morris Street
Oxford, MD 21654

-----Original Message-----
From: []
Sent: Wednesday, January 19, 2000 5:57 PM
Subject: Problems w/cutting monkey brains

I am having trouble with wrinkles.
I am cutting monkey brains and using the large block cassetts , also the
larger micro poly + slides. When placeing the section on the water bath the
wrinkles won't leave the outer edges of the tissue. I even tried putting a
drop of 95% alc in the waterbath, this did help a little but not enough.
tried to cut at different thickness. The pathologist wants no wrinkles
because she want to take pictures of theses slides.
Are there any ideas on what to try? Also what setting would you set the
waterbath at
ours is set at 38 degrees.
Thank you
Dept Government Research

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