RE: Problems w/cutting monkey brains

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From:"RODRIGUEZ, RICHARD" <> (by way of Marvin Hanna)
Content-Type:text/plain; charset="us-ascii"

     Try raising your water bath temp to 45, but you must pick up the
     section quickly before the heat breaks up the tissue.  For H+E's cut
     at 4-5 microns. Try putting more than a drop of alcohol, a few ml's
     may work better.

     Richard Rodriguez
     Schering-Plough Research Institute

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Subject: Problems w/cutting monkey brains
Author:  "" [] at S-P_EXHUB_AM
Date:    1/19/2000 5:56 PM

I am having trouble with wrinkles.
I am cutting monkey brains and using the large block cassetts , also the
larger micro poly + slides. When placeing the section on the water bath the
wrinkles won't leave the outer edges of the tissue. I even tried putting a
drop of 95% alc in the waterbath, this did help a little but not enough.
tried to cut at different thickness. The pathologist wants no wrinkles
because she want to take pictures of theses slides.
Are there any ideas on what to try? Also what setting would you set the
waterbath at
ours is set at 38 degrees.
Thank you
Dept Government Research

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