>From:	Robert Schoonhoven [SMTP:rschoonh@sph.unc.edu]

> >APES for Immuno, Chrome Alum for many of the harsher
> >histochemistries
>
> I used to use chrome-alum for mounting thick (75um) sctions of brain,
> and then clean, uncoated slides for immuno on frozens.
>
> Now, i am doing insitu, and immuno on frozens, i have started using
> home made APES coated slides pretty much as standard.....
>
> My boss wanted me to buy poly-l-lysine slides, in order to save me
> time, but i think i won the battle.......i found that the thick brian
> sections didnt stick too well on them, whilst i dont seem to have any
> problem with the APES ones.....
>
> emma carter
>