I have used a solution called Bouin's Substitute from IMEB for the past 2
years and it works wonderfully.

-----Original Message-----
From: Reynolds, David  A
To: Hagerty, Marjorie A.; 'Histonet'
Sent: 1/23/00 3:47 AM
Subject: RE: Bouins Substitute?

I have an old Histonet message that indicates Weigert's iodine may be
used
in place of Bouin's.  Apparently three minutes at room temperature in
Weigert's yields good results, although I have not tried this.

> -----Original Message-----
> From:	Hagerty, Marjorie A. [SMTP:mhagerty@emc.org]
> Sent:	Friday, January 21, 2000 1:02 PM
> To:	'Histonet'
> Subject:	Bouins Substitute?
>
> I read an article that said it was no longer necessary to use Picric
Acid
> in
> the histo lab. Do any of you substitute the Bouins in the Trichrome
Stain?
> Can it be done?
>
> Thanks,
> Marg
>
> Eisenhower Medical Center
> 39-000 Bob Hope Drive
> Rancho Mirage, CA 92270
>