I have been performing this technique on mouse and guinea pig tissue with
beautiful results!  I am assuming you post-mordanted in Bouin's?

Jeanine H. Bartlett, HT(ASCP)
Centers for Disease Control
NCID, DVRD, IDPA
1600 Clifton Rd., NE  MS/G-32
Atlanta, GA  30333


-----Original Message-----
From: denise M m Long-Woodward [mailto:denisew2@juno.com]
Sent: Thursday, January 27, 2000 3:13 PM
To: emry@u.washington.edu
Cc: HistoNet@pathology.swmed.edu
Subject: Re: Apop/trichrome


I had the same experiences with my mouse tissues. The green was way too
dark.
Denise Long Woodward

On Thu, 20 Jan 2000 18:14:56 -0800 (PST) "P. Emry"
<emry@u.washington.edu> writes:
> Hi all,
>
> I have tried the apop modified trichrome technique written up in the
> Dec
> 99 Histotech Journal.  Done exactly as written I have extreme
> over-staining of the second (green) stain. I have it down to 7 1/2
> mins
> and still get some over-stain.  Is there something I'm missing?
> Should I dilute the stain instead of cutting down on time?
>
> I need a surefire apop technique if there are any others out there
> please
> let me know.
>
> Thanks,
>
> Trisha
> uw seattle
>
>

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