Osmium in Immuno

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From:"Mark Elliott" <MElliott@mrl.ubc.ca> (by way of Marvin Hanna)
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Someone was asking about using Osmium tetroxide to darken DAB
labelling.  When I was at U of Toronto, we used to use 1% OsO4 for 1-2
minutes.  We would wash the DAB off the slides with water and then place
1-2 drops of OsO4 onto each slide in a fume hood, and the wash well with
water.  It gave soem background staining but since we didn't counterstain
our sections it helped us to identify the tissues and made photography
better.  It was very easy to tell the difference between the positive stain
the background.


Mark Elliott, PhD
Research Associate
UBC-Pulmonary Research Lab
St. Paul's Hospital
Vancouver, BC Canada V6Z 1Y6
604-806-8351 (FAX)

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