Kentucky Society for Histotechnology Symposium

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From:"Renee C. Matherly" <> (by way of Marvin Hanna)
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Attention Histonetters
"Looking Into The Future"
Kentucky Society for Histotechnology
24th Annual Symposium
March 17&18,2000
Holiday Inn Downtown
120 W. Broadway

Friday, March 17, 2000

Session I
8:00-9:00  Bioterrorism: The Role of the Clinical  Microbiology
9:00-9:30       Break with Vendors
9:30-10:30     These Bones Werenit Made  Just For Walking
10:30-11:00  Break with Vendors
11:00-12:00  The Evolving Role of Histotechnology  in Emerging
Infectious Diseases
Session II
8:00- 12:00 Troubleshooting the  Immunohistochemical Procedure
  (Break with Vendors 10:00-10:30)

12:00-1:00  Lunch

Session III
1:00-2:30  Flow Cytometric Analysis in the Diagnosis of Leukemia and
2:30-3:00  Break with Vendors
3:00-4:00  PCR in the Clinical Laboratory: Current and Future
Session IV
1:00-4:00  US FDA Good Laboratory Practice  Requirements for
Nonclinical  Studies
(Break with Vendors 2:30 - 3:00)

Saturday, March 18, 2000

Scientific Sessions

8:00-9:00  Carpal Tunnel Syndrome
9:00-10:00  Histopathologic and Immunofluores  cence Pattern of
Immunologic Bullous Disorders
10:00-10:30 Break with Vendors
10:30-11:15 Electron Microscopy: General Overview  and Current Utility
11:15-12:15 The Peripheral Nerve Biopsy
12:15-1:15  Lunch
1:15-2:15  Genetic Susceptibility to Cancer:   iVinyl Chloride-Inducedi
2:15-2:45  Histology Waste:  What To Do??!!
2:45-3:15   Break with Vendors
3:15-4:00  Panel Discussion:   Answering Your Questions

Course overview
Bioterrorism: The Role of the Clinical Microbiology Laboratory
James W. Snyder, PhD(ABMM); Professor of Pathology
Director, Microbiology, University of Louisville
Learning Objectives:  1.  List the 5 Biological Agents targeted by NATO
anticipated to be used in an act of bioterrorism. 2.  Describe why
biological agents are adaptable for use as weapons in acts of
bioterrorism.  3.  Identify and characterize the respective levels of
biosafety.  4.  Describe the structure and function of the proposed
laboratory network system designed to detect and confirm the identity of
a biological agent.

These Bones Weren't Made Just for Walking
Scott C. Miller, PhD; Director, Research Professor Radiobiology.
University of Utah.  A histological tour of the skeletal system in a
variety of circumstances will be conducted.  Our tour will include a
visit to a poultry farm, outer space, a gym, geriatric center and a
plutonium plant. A variety of histological techniques and approaches
used in the skeletal sciences will be presented.

The Evolving Role of Histotechnology in
Emerging Infectious Diseases
Tim Morken, BA, EMT(EMSA),HTL(ASCP); Division for Infectious Disease,
Centers for Disease Control and Prevention Histotechnology plays a role
in the laboratory which is complimentary to microbiology and serology.
While diagnostic work can be done in either, it is only in the
histology lab that the morphological pathogenesis of the disease can be
determined.  Diagnosis and research of infectious diseases by
histotechnology now runs the gamut from the "special stains" to
molecular  biology.  The use of this broad range of technologies in the
histology laboratory at the Centers for Disease Control will be

Troubleshooting the Immunohistochemical Procedure; Emphasis on Technical
and Interpretative
Sheron C. Lear, HT/HTL(ASCP), QIHC; Supervisor, Special Procedures
Laboratory, University of Louisville Alvin W. Martin, MD; Medical
Consultant, Special Procedures Laboratory, Director Flow Laboratory,
University of Louisville. Immunohistochemistry, a mainstay in diagnostic
pathology, must be efficient, rapid and reproducible, although there are
numerous pitfalls.  Procedures that we consider routine, such as
fixation and processing, can have adverse effects on certain antigens.
Other considerations to be addressed are removal of endogenous pigments,
antigen unmasking techniques, fixation and isotype controls, selection
of antibodies, lineage of cell in question and pattern of antibody
reactivity, and competition studies for antibody-antigen correlation.
Bring your problems and concerns.

Flow Cytometric Analysis in the Diagnosis of Leukemia and Lymphoma
Karen M. Cost, Ph.D; Chief of Immunology,  Norton Healthcare, Inc.
Information obtained by flow
cytometric analysis on bone marrow aspirates and lymph node biopsies is
critical in making the
diagnosis of leukemia or lymphoma, and in determining appropriate
therapy.  Principles of the
technique of flow cytometry will be reviewed, along with data about the
classification of lymphoid
malignancies.   Selected case studies that emphasize the contribution of
flow cytometry will be presented.

PCR in the Clinical Laboratory: Current and Future Applications
Robert L. Barker, Ph.D; Assistant Professor (Clinical Services),
Department of Pathology and Laboratory Medicine, University of
Louisville.  Polymerase chain reaction (PCR) technology has
revolutionized molecular biological  techniques by greatly increasing
our ability to unravel and catalog the information contained in the
human genome.  Current PCR technology is very labor intensive.
Automated PCR systems have been developed and show promise, but their
use is limited at this time.  Technologies are being developed that
hopefully will allow an individual to be screened for tens of thousands
of DNA targets at a time.  This should lead to better understanding of
biology and the clinical needs of the individual patient and thus result
in better patient care that is
more tailored to the specific needs of the individual.

US FDA Good Laboratory Practice Requirements for  Nonclinical Laboratory
Allen W. Singer, DVM; Director of Operations,
 Toxicology Columbus, Battelle, Columbus, OH
An overview of the processes required to register new drugs in the
United States will be presented.
The creation of federal laws pertaining to various nonclinical studies
will be outlined,including why the
regulations were created and to whom they pertain.  The intersection of
these regulations and the
work of histologists and necropsy technicians will be covered in detail,
to include various necropsy
techniques and data collection requirements under the Good Laboratory
Practices Regulations (21 Code of Federal Regulations, Part 58).  Data
will be presented from a variety of studies to demonstrate how
veterinary pathology is often a critical factor in the drug approval

Carpal Tunnel Syndrome
Douglas T. Kane, D.C.; Kane Chiropractic
Causes and Prevention:  Definition, history, anatomy, causes, treatment
and prevention.

Histopathologic and Immunofluorescence
Pattern of Cutaneous Immunologic Bullous Disorders
Vilma C. Fabre', MD; Dermatopathology Consultants, P.S.C.
A discussion of the routine histopathologic findings and the
imuno- fluorescence pattern of autoimmune bullous disorders of the skin.
This will include a discussion of the circulating (serum) imunoglobins
directed against the components of the basal cell layer keratinocytes
and basement membrane zones.  There will be a strong emphasis on
clinical features as they present in the skin of  the patients.
Photomicrographs and clinical photos will be used.

Electron Microscopy: General Overview & Current Utility
Lisa Wills-Frank, MD; Associate Professor; University of Louisville.
Basic structure and function of the electron microscope will be
discussed.  Case examples in which ultrastructural examination may be
useful and important in making the diagnosis.

The Peripheral Nerve Biopsy
Jewel I. Rice, BS, HT(ASCP); Hamilton County Coroner's Office,
Cincinnati, Ohio.
Receiving, gross description, freezing, dissection, fixation,teasing and
histology of the peripheral nerve biopsy specimen in the clinical
laboratory. The indentification of common diseased fibers in the nerve
biopsy specimen and the mounting of fibers on slides for diagnosis.

Genetic Susceptibilty to Cancer:"Vinyl Chloride-induced"
Deborah Antonino-Green, MT(ASCP), PhD; Fellow,
Clinical Chemistry and Toxicology; Department of Pathology and
Laboratory Medicine, University of Louisville.
Many diseases that affect us today will be avoidable or treatable in
years to come by evaluating a personis genetic susceptibility to
specific disease- causing agents. Ever-increasing awareness in
preventive medicine demands the clinical laboratory to pay close
attention to basic genetic research and evaluate the possible clinical
applications for this information.  The wealth of knowledge gained from
genetic research will someday be used for risk stratification in a role
in of genetic variation in occupational and/or environmental medicine
and the clinical laboratory.

Histology Waste:  What To Do??!!
Barbara Beckman, HT/HTL(ASCP); Supervisor, Histology, LabCorp
Louisville,Kentucky.  Kentucky regulations for the discarding of
histology generated solutions will be discussed.  Proper procedure and
handling of hazardous waste, recycling of alcohol and xylene, pros and
cons.  Discussion of individual laboratory's procedures.

Panel Discussion:  Answering Your Questions
Mary Beth Knight, BS, MT/HTL(ASCP); Supervisor, Pathology, Norton
Healthcare, Inc.Terry Lacy, HTL(ASCP),
Sheron C. Lear, HT/HTL(ASCP),QIHC;Supervisor, Special Procedures
University of Louisville Ela Patel, HT(ASCP), QIHC; Sanders-Brown,
Center on Aging University of Kentucky.
This open forum will give histotechs an opportunity to troubleshoot some
of their persistent problems.  Please bring concerns and helpful Hints

The Kentucky Society For Histotechnology
24th Annual Symposium
Advanced Registration
(Due March 10, 2000)

City, State, Zip: _______________________________
Email: _______________________________________

Workshop Friday, March 18, 2000
AM                  I   II             (circle one)
PM         III                 IV            (circle one)
KSH member $25.00 each workshop
Non-member  $35.00 each workshop
Saturday March 18, 2000
______ Scientific Sessions
KSH member $35.00 each workshop
Non-member  $45.00 each workshop
Make checks payable to:
Kentucky Society for Histotechnology
Send registration form and checks to:
 Jean Lambert
1545 McKay Avenue
Louisville,Ky 40213


 This program is designed to provide continuing education to
histologists and pathologists from hospitals and research laboratories.
The objectives is to improve standards in histopathology laboratories
with up-to-date methods and scientific advances in the field of
histotechnology.  Outstanding faculty have been selected.    Ample time
will be allotted for participants to view the latest equipment and talk
with representatives from the various companies.

 Holiday Inn  Downtown at 120 W. Broadway, Louisville, Kentucky.  Make
reservations by calling (502) 582-2241 or  800-626-1558.

Early Registration is requested.
Workshop/Per Workshop
 KSH member         $ 25.00
 Non member / late registration        $ 35.00

Scientific Sessions per day
 KSH member         $ 35.00
 Non member / late registration                   $ 45.00
This fee includes Friday and Saturday luncheon, coffee breaks and
hospitality reception on Friday with exhibitors.

CMLE Credit:
 This  symposium is recognized by the American Society of Clinical
Pathologists as meeting the criteria for 12 hours of credit
Category A1 for the Recognition Award for CMLE.  The CMLEis will be
awarded to those attending each session. To receive CMLE credit, it is
necessary to complete the application for each session attended and
return it to the KSH liaison at the end of the session.

From East or West I-64 proceed to I-65 South.
From South I-65 .  Exit at Jefferson Street. At Jefferson and 1st street
turn left.  Continue on 1st street until it intersections with
Broadway.  Turn right on Broadway.  Holiday Inn is 1/2 block on the
From North I-65:  Exit on Broadway.  Turn left at the light. Holiday Inn
will be 2 blocks on the left.

Additional Information:
Contact Reneei Matherly,President,KSH,4312 Conaem Drive, Louisville,KY
w502/852-5587,h502/459-5350,f502/852-1767, email RMath0516@AOL.COM

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