(Fwd) Mail delivery failed: returning message to sender

<< Previous Message | Next Message >>
From:"RUSS ALLISON" <Allison@Cardiff.ac.uk> (by way of Marvin Hanna)
To:histonet@histosearch.com
Reply-To:
Content-Type:text/plain; charset="us-ascii"




Subject: Re: paraffin
Reply-to: ALLISON@CARDIFF.AC.UK

LuAnn,
Unless you have a specific purpose, there is little to gain by using
a different wax for embedding to that used for infiltration.  If you are
having trouble cutting sections (and are not one of those trying to
perform miracles by cutting turtle shells etc.), 99.5% of problems
will be associated with the knife or its setting rather than the
embedding wax.

I am making the the massive assumption that the rest of the
processing is good.

If you do want to embed in a different wax, the procedure is simple:-
just do it.  Only real precaution is to ensure infiltration wax soaked
tissue has not cooled too much before embedding so that you
avoid an obvious interface between the two waxes.

I have used this method for bone and other hard/brittle tissues.

There is no doubt that some paraffin waxes have better infiltration
properties than others and some which have better sectioning
properties.

This presumes that you are not talking about "double embedding",
a description usually reserved for the sequence of paraffin/celloidin
embedding.


Russ Allison,
Dental School
Cardiff
Wales
------- End of forwarded message -------
Russ Allison,
Dental School
Cardiff
Wales




<< Previous Message | Next Message >>