Frozen tissue efficacy
<< Previous Message | Next Message >>
From: | Susan Q Wells <susan.wells@bms.com> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
I have frozen panels of tissue from
dogs,mice,rats,rabbits,cyno's,and humans . I have been asked to
do an immunologic stain to test the efficacy of these stored
tissues. They are from 5 years old to present. I don't
necessarily have to do the same stain on all the species but I
don't want this to get too crazy. My first thought was vimentin
but I can't find one that cross reacts.
I have some experience with B cell staining so am considering
pulling the lymphoid tissue and staining for them I can't seem to
find an anti -rabbit reagent or an anti -mouse reagent thats been
tested on cryostat sections. Cyno's are no problem,I can use anti
humans reagents there. I found canine and rat reagenta at
Serotec. I use a Ventana ES and have the capability of using it
fairly openly. I prefer purified reagents but biotinylated would
be OK too. Anyone out there have any suggestions on
reagents/vendors or a way to keep this simple and answer all my
questions. Of course I'll do an H&E to check morphology first.
Thanks in advance,and I'll be happy to share any info I get out
of this project.
<< Previous Message | Next Message >>