> ----------
> From: 	Molinari, Betsy
> Sent: 	Thursday, January 20, 2000 6:40 AM
> To: 	'Kathy Wortham'
> Subject: 	RE: adhesives
>
> Another silane protocol:
>  Be sure slides are very clean to begin with, we wash ours in
> detergent,(can be anything from Joy to Alconox but a only a bit of
> detergent). Then rinse well in tap H20 then in DH20.
> Use only glass slide racks, silane is corrosive and will ruin your
> metal ones.
> 1) dip in 50/50 acetone/ DH20 solution 5-6 times
> 2)place in 3-aminopropyltriethoxy silane solution (6ml of silane to
> 300 ml of acetone) for 5 min.
> 3)dip in 100% acetone 5-6 times
> 4) dip in 2nd 100% acetone 5-6 times
> 5) dip in DH20 5-6 times
> 6) dip in 2nd DH20 5-6 times
> we dry in oven at 58-62 degrees, but they can be dried overnight at
> room temp.
> The 3-aminopropltriethoxy silane sol is good for about 200- 250 slides
>
> Betsy Molinari
> Texas Heart Institute
> Cardiovascular Pathology
> Houston, Texas
> 713-794-6524
> bmolinari@heart.thi.tmc.edu
> ----------
> From: 	Kathy Wortham[SMTP:kjwortham@yahoo.com]
> Sent: 	Wednesday, January 19, 2000 3:09 PM
> To: 	Maria Mejia
> Cc: 	HistoNet Server
> Subject: 	Re: adhesives
>
>
>
> --- Maria Mejia <maria@mail.ski.org> wrote:
> > Hello, can you please send me the protocol for
> > making APES.
> > Thank you in advance.
> > maria mejia
> > smith-kettlewell eye res. inst.
>
>
> Procedure for Silane coated slides
>
> Materials:
> Silane  (3-aminopropyltriethoxysilane)
> Acetone
> Distilled Water
> Slides
> Staining racks
> Staining dished
>
> Procedure:
> 1--Place slides in staining racks
> 2--Acetone--2 minutes
> 3--2% Silane solution  (5ml Silane to 250ml Acetone)
> 4--Distilled water--5 dips
> 5--Distilled water--5 dips
> 6--Distilled water--5 dips
> 7--Allow slides to air dry at room temp overnight
>    (does better than drying in oven)
> 8--Place slides in slide box
>
>
>
> =====
> Kathy Wortham
> USDA,Food Safety Inspection Service
> 950 College Station Road
> Athens, Ga 30605
> (706)546-3556
> kjwortham@yahoo.com
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