FW: Apop/trichrome
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From: | "Rosebury, Wendy" <Wendy.Rosebury@wl.com> (by way of Marvin Hanna) |
To: | histonet@histosearch.com |
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Content-Type: | text/plain; charset="us-ascii" |
Hi Jeanine,
Did you do the technique on formalin or Bouins fixed tissues?
Wendy Rosebury
Parke Davis
-----Original Message-----
From: Bartlett, Jeanine [mailto:jqb7@cdc.gov]
Sent: Friday, January 21, 2000 6:33 AM
To: 'P. Emry'; HistoNet@pathology.swmed.edu
Subject: RE: Apop/trichrome
I have just tried the same technique and did not have overstaining. I am
using it at the full 30 minutes and am very careful not to linger in water
or alcohol as I do not want to lose any of the stain. I would simply make
fresh light green and try it again at 30 minutes. Perhaps there was a
simple miscalculation that you are unaware of.
Good luck!
Jeanine H. Bartlett, HT(ASCP)
Centers for Disease Control
NCID, DVRD, IDPA
1600 Clifton Rd., NE MS/G-32
Atlanta, GA 30333
-----Original Message-----
From: P. Emry [mailto:emry@u.washington.edu]
Sent: Thursday, January 20, 2000 9:15 PM
To: HistoNet@pathology.swmed.edu
Subject: Apop/trichrome
Hi all,
I have tried the apop modified trichrome technique written up in the Dec
99 Histotech Journal. Done exactly as written I have extreme
over-staining of the second (green) stain. I have it down to 7 1/2 mins
and still get some over-stain. Is there something I'm missing?
Should I dilute the stain instead of cutting down on time?
I need a surefire apop technique if there are any others out there please
let me know.
Thanks,
Trisha
uw seattle
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