FW: Apop/trichrome

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From:"Rosebury, Wendy" <Wendy.Rosebury@wl.com> (by way of Marvin Hanna)
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Hi Jeanine,
Did you do the technique on formalin or Bouins fixed tissues?

Wendy Rosebury
Parke Davis

-----Original Message-----
From: Bartlett, Jeanine [mailto:jqb7@cdc.gov]
Sent: Friday, January 21, 2000 6:33 AM
To: 'P. Emry'; HistoNet@pathology.swmed.edu
Subject: RE: Apop/trichrome

I have just tried the same technique and did not have overstaining.  I am
using it at the full 30 minutes and am very careful not to linger in water
or alcohol as I do not want to lose any of the stain.  I would simply make
fresh light green and try it again at 30 minutes.  Perhaps there was a
simple miscalculation that you are unaware of.

Good luck!

Jeanine H. Bartlett, HT(ASCP)
Centers for Disease Control
1600 Clifton Rd., NE  MS/G-32
Atlanta, GA  30333

-----Original Message-----
From: P. Emry [mailto:emry@u.washington.edu]
Sent: Thursday, January 20, 2000 9:15 PM
To: HistoNet@pathology.swmed.edu
Subject: Apop/trichrome

Hi all,

I have tried the apop modified trichrome technique written up in the Dec
99 Histotech Journal.  Done exactly as written I have extreme
over-staining of the second (green) stain. I have it down to 7 1/2 mins
and still get some over-stain.  Is there something I'm missing?
Should I dilute the stain instead of cutting down on time?

I need a surefire apop technique if there are any others out there please
let me know.


uw seattle

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