CD3 has too much background, ca you help?

<< Previous Message | Next Message >>
From:"Sandy Julsing" <> (by way of Marvin Hanna)
Content-Type:text/plain; charset="us-ascii"

     Our CD3 has too much background stain including in the gastric glands.
Our protocol is...

-DAKO pre-dilute CD3
-antigen retrieval with citrate buffer pH 5.8-6.2 for 20 min with a 20 min
cool down
-LSAB2 detection kit 10min/10min/5min
-Stained on the DAKO autostainer

 Any help would be greatly appreciated, thanks,  Sandosis

<< Previous Message | Next Message >>