CD3 has too much background, ca you help?
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| From: | "Sandy Julsing" <sandy.julsing@thedacare.org> (by way of Marvin Hanna) |
| To: | histonet@histosearch.com |
| Reply-To: | |
| Content-Type: | text/plain; charset="us-ascii" |
Histo-buddies
Our CD3 has too much background stain including in the gastric glands.
Our protocol is...
-DAKO pre-dilute CD3
-antigen retrieval with citrate buffer pH 5.8-6.2 for 20 min with a 20 min
cool down
-LSAB2 detection kit 10min/10min/5min
-Stained on the DAKO autostainer
Any help would be greatly appreciated, thanks, Sandosis
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