Hi all,

I have tried the apop modified trichrome technique written up in the Dec
99 Histotech Journal.  Done exactly as written I have extreme
over-staining of the second (green) stain. I have it down to 7 1/2 mins
and still get some over-stain.  Is there something I'm missing?
Should I dilute the stain instead of cutting down on time?

I need a surefire apop technique if there are any others out there please
let me know.

Thanks,

Trisha
uw seattle