10% NBF for TEM fixation?

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From:Carla_Aiwohi@usgs.gov (Carla Aiwohi) (by way of Marvin Hanna)
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Happy New Year everyone-

Recently we received whole #003#zebrafish mistakenly fixed in 10% NBF.  We are
interested in examining muscle with TEM and usually fix tissues in modified
Karnovsky's then post-fix in 1% osmium tetroxide.  Would it be better to post-
fix directly from NBF or should I re-fix (?) in Karnovsky's and carry on as
usual?  Also, why isn't 10% NBF suggested as a primary fixative for EM?

Thanks for your help-

Carla Aiwohi
Western Fisheries Research Center
Seattle, WA

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