re: processing rodent skin
|From:||"Philopena, Jennifer" <firstname.lastname@example.org>|
I am trying, without much success, to get mouse and rat skin processed so
that the samples will section without blowing out or disintigrating in the
water bath after sectioning. I have a Shandon Citadel processor and have
noticed that the problem improves when the processing reagents are fresh
(that's a lot of reagent to change out!) The skins are fixed for at least 2
days in 10% formalin, and the mouse skin is processed on a 15 minute per
reagent schedule (70%, 80%, 2x95%, 2x100%, 3xPropar, 2 wax baths). The rat
skin is processed on a 30 minute per reagent schedule. The skin is kept
flat in the cassettes using rectangular sponges.
Any input would be so appreciated!
Thanks in advance.
Jennifer M. Philopena
3525 John Hopkins Court
San Diego, CA 92121
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