"Venetian Blind" Artefact
|From:||Stephanie Moore <firstname.lastname@example.org>|
I have recently experienced what I believe to be this artefact while slicing
sections of squirrel brain. I know that this was not properly sunk and also that
it was likely in too little volume of paraformaldehyde (4%) fixative. The person
who gave me the specimen is not very experienced, but wanted to slice it anyway.
My question is, does insufficient cryoprotection from sucrose cause ventetian
blinds? I use a cryostat set at -20C chamber temperature and -18C object
temperature, and Tussue Tek OCT medium for embedding the specimen.
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