double IHC/in situ

From:Karen Larison <>

Hello Histonetters,

Some of the researchers around here do in situ hybridization followed 
by IHC.  However, the harsh conditions of the in situ hybridization 
techniques destroys many of the antigenic sites of interest.  Does 
anyone out there use antigen retrieval to recover antigenicity 
following in situ techniques, or does anyone know of any references?

Karen Larison
University of Oregon

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