Which H&E?

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From:<larisonk@uoneuro.uoregon.edu> (Karen Larison) (by way of histonet)
To:histonet@histosearch.com
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Hello Netters,

I'm trying to brighten up my H&E stain.  It's been suggested that I switch
from
Gill's #2 hematoxylin to Harris' hematoxylin.  I'm hesitant to do this, simply
because I'm loathe to use mercuric oxide if it can be avoided.  Will this
change make
a big difference in the quality of my staining?  (I'm staining adult fish).

Also, it's been recommended that I switch to an eosin containing a higher
percentage
of alcohol.  This is no problem, but are there problems I might encounter
by making
this switch?

Karen in Oregon









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