Which H&E?
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From: | <larisonk@uoneuro.uoregon.edu> (Karen Larison) (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Hello Netters,
I'm trying to brighten up my H&E stain. It's been suggested that I switch
from
Gill's #2 hematoxylin to Harris' hematoxylin. I'm hesitant to do this, simply
because I'm loathe to use mercuric oxide if it can be avoided. Will this
change make
a big difference in the quality of my staining? (I'm staining adult fish).
Also, it's been recommended that I switch to an eosin containing a higher
percentage
of alcohol. This is no problem, but are there problems I might encounter
by making
this switch?
Karen in Oregon
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