What stain to replace the H&E??
|From:||Tony Henwood <AnthonyH@chw.edu.au>|
John Kiernan recently commented:
"H & E is popular because pathologists (who rarely do the staining
themselves) have for many years considered it the "routine" or
"standard" technique. There are plenty of other staining methods
that are easier to do, do not require microscopic control to get
the best results, use cheaper chemicals, and provide more informative
colour schemes in the sections. Research workers (who often have to
do their own staining and teach the art to graduate students and new,
untrained technicians) often prefer methods that are both technically
simpler and more informative than H & E."
This got me thinking (a dangerous pursuit!!). What stain would histotechs
recommend to replace the H&E??
I have some fondness for John's "Iron-Chromoxane Cyanine R".
Any other ideas??
Tony Henwood JP, BappSc, GradDipSysAnalys, CT(ASC)
The Children's Hospital Westmead,
Locked Bag 4001, WESTMEAD, 2145.
Tel: (02) 9845 3306
Fax: (02) 9845 3318
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