Mary wrote:
<Inclusion bodies etc are the result of changes caused by
the virus rather than the virus itself.>

The inclusion bodies you see by light microscopy are large concentrations of
virus which sometimes can be crystalline arrays.

Tim Morken, B.A., EMT(MSA), HTL(ASCP)
Infectious Disease Pathology
Centers for Disease Control
MS-G32
1600 Clifton Rd.
Atlanta, GA 30333
USA

email: tim9@cdc.gov
       timcdc@hotmail.com

Phone: (404) 639-3964
FAX:  (404)639-3043


----Original Message Follows----
From: Mary Latimer <ml4@st-andrews.ac.uk>
To: mark.lewis@shandon.com
CC: Histonet@pathology.swmed.edu
Subject: Re: May-Grunwald
Date: Thu, 6 Jan 2000 14:02:21 +0000 (GMT)

Please correct me but I always assumed that a virus was too small for
light microscopy.Inclusion bodies etc are the result of changes caused by
the virus rather than the virus itself.Mary

On Wed, 5 Jan 2000 mark.lewis@shandon.com wrote:

 > Happy New Year everyone!
 >
 > I'm not very familiar with the use of Wright-Giemsa and May-Grunwald
stains.  I've had limited experience in using these two stains.
 > I understand that these two stains are two different staining
formulations, but  I've been asked to find out if The May-Grunwald  stain
will demonstrate viruses in any  form in paraffin sections.  If it does what
is the expected result ( how will it be demonstrated) ?  Does it demonstrate
Viral Inclusion bodies of only some viruses and not others?  I know a Wright
Giemsa will demonstrate various parasites and bacteria on a blood smear, but
do not know if it demonstrates viruses in Paraffin tissue sections.
 > The person I'm asking for is a customer that is specifically looking for
Adenovirus.  I've suggested they find a vendor that sells the Adenovirus
antibody, but they seem intent on using May-Grunwald.  If M-G works, would a
regular Wright Giemsa work ?
 >
 > Your Knowledgeable input is ,as always, very much appreciated.
 >
 > Thanks
 >     :)
 > Mark Lewis
 >
 >
 >








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