Re: shrinkage in the dehydration of mouse brains

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From:"Sarah Christo" <schristo@cvm.tamu.edu> (by way of histonet)
To:histonet@histosearch.com
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If you don't want it to shrink, don't put it in alcohol.  The brain is
mostly water.  Some plastics can infiltrate from water, but I think some
shrinkage still happens.  Sarah
Also, shrinkage can be avoided by not swimming in cold water.  Seinfeld

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
Histology Laboratory
College Station, TX  77868-4458
schristo@cvm.tamu.edu
phone (409) 845-3177
fax (409) 847-8981

>>> Peter Poon <peterp@cafe.berkeley.edu> 01/10 11:51 AM >>>
hi all, I have been working on the dehydration of mouse brains, but I have
been getting about a 50% shrinkage on overall volume. I have been using a
ethanol/isopropyl dehydration series. Does anyone have any ideas on how to
prevent shrinkage? Please help . Thanks


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