Re: profusion

From:"John C. Dennis" <>


Take the fetuses out and fix by immersion.  Recall the blood/placental
barrier.  The placenta is embryonic tissue and receives from the
mother/dumps to the mother metabolites, oxygen, etc.  The fetus has its
own blood supply so you can't bleed/flush the vascular system by
perfusing the mother.  I just had to try perfusing the mother just to 
see.  It worked real well except that none of the fetuses were fixed.

Yours in separation

John Carroll Dennis
Anatomy, Physiology, and Pharmacology
109 Greene Hall
Auburn University, AL  36849

On Wed, 3 Feb 1904, Colleen Forster wrote:

> Histonetters,
> Here is a question on profusion.. if you would profuse a pregnant mouse
> would the para penerate the placenta and fix the embryos or do you need
> to remove them first and drop fix ?
> Currently we anesthetize the female and then remove the embryos but the
> above question was raised at the last lab meeting and I couldn't answer
> it.
> As always thanks in advance for any help.
> Colleen Forster
> U of MN, Dept. of Neurology

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