This is what I have:

Michels Buffer
1M potassium citrate, pH 7.0            2.5 ml
0.1M magnesium sulfate                   5.0 ml
0.1M N-ethyl malemide                      5.0 ml
d H2O                                                 87.5 ml
* Mix well and store at 2-8C.   Exp. 1 year

Michel's Fixative
Michel's Buffer                                    100ml
ammonium sulfate                                55gm
Adjust pH to 7.0-7.4 with 1M KOH.  Store at 2-8C.  Exp. 1 year

Bonnie Whitaker
UT--Houston

----- Original Message -----
From: Atoska Gentry <gentras@vetmed.auburn.edu>
To: <histonet@pathology.swmed.edu>
Sent: Friday, January 12, 2001 11:50 AM
Subject: michele's fixative


> Please will someone  provide me with a recipe for michele's fixative used
> for immunoflourescence ASAP?  Thanks, Atoska
>
> Atoska  S. Gentry B.S., HT(ASCP)
> Research Assistant II
> Scott-Ritchey RSCH Center
> Auburn University, AL  36849
> PH:   (334) 844-5579
> Fax:  (334) 844-5850
>
>