i infuse with sucrose for 2 hours at room temp, and it has worked fine so far.

i have also been told of an alternative procedure where the samples are
infused with a sucrose/PVP mixture. Does anyone know why PVP is used? Is it
any better as a cryoprotectant? any possible problems?

thanks a bunch,
-Tahir


At 02:53 PM 1/15/01 -0600, Jeneile Marlow wrote:
>Hi, I am doing cryo sections on animal tissue and will be doing snap
>freezing  with liquid nitrogen. The tissue specimens will be fixed before
>hand in 4% Para formaldehyde, I do not want to store in sucrose overnight
>because of the potential for interference with immunohistochemistry. Does
>anyone have any ideas on what I can use as substitute? Thanks Jenny.
>