Re: Substance P

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From:Dana Settembre <settembr@UMDNJ.EDU> (by way of histonet)
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On Tue, 11 Jan 2000, Sabourin, Carol L wrote:

> Has anyone used a substance P antibody in their IHC studies?  I want to do
> IHC for substance P in mouse skin. Transduction/PharMingen has a rat
> monoclonal that has been used on paraformaldehyde-fixed, frozen sections of
> rat brain. They state that the antibody reacts with mammalian substance P.
> Their technical rep said that they find proteins expressed in the brain to
> be fragile and most of the literature they review for these reagents uses
> frozen sections.  I want to use formalin-fixed, paraffin-embedded tissues. I
> fix for 24 hr and then transfer to PBS at 4C until the tissue is processed.
> I have found that it is important not to "over fix" for many antibodies.
> Also, does anyone use the TSA signal amplification system from NEN in their
> IHC protocols?
> Carol L. Sabourin, Ph.D.
> Battelle
> Columbus, OH
> Phone: (614) 424-5024
> Fax: (614) 424-5469
Hello Carol
I have used Substance P from 	Arnel Products Co. Inc.
				P.O. Box 20045
				NY, NY  10021
Their cat# 1768
I used it at a 1:3000 dilution with a 5 min prot. K pretreatment with a
brain as a control.
good luck.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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