Re: Sausage Casing

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From:Gwendolyn Goldberg <bonnimus@yahoo.com> (by way of histonet)
To:histonet@histosearch.com
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Thanks bunches....sounds like a great idea to me.

--- "Shotsberger-Gray, Wanda"
<WandaShotsberger-Gray@hmhs.com> wrote:
> Lynn and Gwen,
> first off sausage blocks are a way of making control
> blocks for immunos that
> have all the tissues for controls in one block, thus
> saving the user from
> having to maintain a huge library of control blocks
> for immuno procedures.
> The sections are "mapped" so that the user knows
> what tissue is where in the
> block, As the tissue is processed, I would imagine
> ithey are rather yucky
> with eggs, but I cannot speak from experience. :->
>   As for sausage casing, Lynn, It may not be
> necessary.  Dr. Rodney Miller
> published a "shortcut" (quite) a few years back in
> the Histo-Logic using
> plain old boring paraffin as a substitute.  If
> memory serves, you cut the
> tissues into matchstick sized pieces, then process.
> After processing, start
> with one piece and roll it in a little paraffin, let
> it get just barely
> hard, take another piece, add it to the first, roll
> both together in the
> paraffin, let 'em get hard enough to stay together
> when you add the third,
> and so on until all the pieces are added.   When
> hardened, you cut the
> "sausage" into embedable sizes, embed and stain to
> map.  I have no idea when
> this was in the Histo-Logic, but doubt it would be
> too hard to find.  Dr.
> Miller cited this article at an immuno session he
> gave at our Texas meeting
> year-before-last.
> Hope this helps, as I have no earthly idea where to
> get sausage casing
> anywhere in the US.
> Wanda Shotsberger
> Harris Methodist Hospital
> Fort Worth TX
>  ----------
> From: Gwendolyn Goldberg
> To: Lynn Gardner; Histonet@pathology.swmed.edu
> Subject: Re: Sausage Casing
> Date: Thursday, January 13, 2000 4:06PM
>
>   Ok..I`ll show my eignorance...what do you do with
> them are they good with eggs????
>
>  --- Lynn Gardner <lynn-gardner@uiowa.edu> wrote:
> > Hey all of you out there in Histoland was
> wondering
> > where people are
> > getting their sausage casing for Multi tumor
> tissue
> > blocks. Would
> > appreciate a quick answer from anyone who knows
> > anything about this. Also
> > it would be helpful if anyone knows where we could
> > get some here in Iowa.
> >
> > Thanks All
> > Lynn Gardner
> >
> >
> >
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