Re: STAIN: Toluidine Blue removal

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From:Geoff McAuliffe <mcauliff@UMDNJ.EDU> (by way of histonet)
To:histonet@histosearch.com
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Jaci Lett wrote:

> Does anyone have a solution for this problem?  I managed to get a spot (<3mm
> around) of Toluidine Blue on the cuff of my new shirt (the only part
> extending beyond my lab coat).  Is there any way to get rid of all (or most)
> of the stain without it spreading?  The shirt is 100% cotton.

Toluidine  Blue is very soluble in ethanol, perhaps even more so with a drop of
acetic acid. If that does not work, maybe lemon juice or oxalic acid.

> If I don't get any response, my inclination is to try a sodium tetraborate
> paste applied with a cotton swab.

    Why sodium tetraborate? I think acid, not alkali, it the way to go.

Geoff
--
**********************************************
Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
675 Hoes Lane, Piscataway, NJ 08854
voice: (732)-235-4583; fax: -4029
mcauliff@umdnj.edu
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