Re: Primary and Secondary Amyloid

From:Rena Fail <>

<html> <font size=3>Tony,<br> <br>        I do use this technique on occasion. Using 47ml. of .5% potassium permanganate and 3 ml of 3% sulfuric acid. .This slides have to stay in this solution for 20 minutes, but there is less problems with tissue washing off. Remember to run a duplicate set of slides without the acidified potassium permanganate.  I use Congo Red not Sirius red because amyloid stained with Congo red will exhibit an orange to red fluorescence .<br> <br>  Amyloid AA loses affinity for Congo Red  after incubation with potassium permanganate. <br> <br>   I have copies of both the references mentioned by John Kiernan.<br> <br>  Rena Fail AS,HT(ASCP)<br>  Medical University of SC<br> Charleston, SC<br> <br> At 12:53 PM 1/4/01 +0000, you wrote:<br> <blockquote type=cite class=cite cite>I have a technique for differentiating between primary and secondary<br> amyloid by treating with a<br> <br> potassium permanganate/sulphuric acid mixture and staining with Sirius Red.<br> Can anyone please tell<br> <br> me if they use this particular technique and let me have any references to<br> who did the modification and<br> <br> where it was published.<br> <br> Many Thanks,<br> <br> Tony Brandwood,<br> Deputy Laboratory Superintendent,<br> Department of Veterinary Pathology,<br> University of Liverpool,<br> P.O.Box 147,<br> Liverpool,<br> L69 3BX.<br> U.K.<br> <br> Tel. No. 0151-794 4251<br> Fax. No. 0151-794 4268<br> e-mail</font></blockquote><br> </html>
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