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<font size=3>Tony,<br>
<br>
I do use this technique on occasion.
Using 47ml. of .5% potassium permanganate and 3 ml of 3% sulfuric acid.
.This slides have to stay in this solution for 20 minutes, but there is
less problems with tissue washing off. Remember to run a duplicate set of
slides without the acidified potassium permanganate. I use Congo
Red not Sirius red because amyloid stained with Congo red will exhibit an
orange to red fluorescence .<br>
<br>
Amyloid AA loses affinity for Congo Red after incubation with
potassium permanganate. <br>
<br>
I have copies of both the references mentioned by John
Kiernan.<br>
<br>
Rena Fail AS,HT(ASCP)<br>
Medical University of SC<br>
Charleston, SC<br>
<br>
At 12:53 PM 1/4/01 +0000, you wrote:<br>
<blockquote type=cite class=cite cite>I have a technique for
differentiating between primary and secondary<br>
amyloid by treating with a<br>
<br>
potassium permanganate/sulphuric acid mixture and staining with Sirius
Red.<br>
Can anyone please tell<br>
<br>
me if they use this particular technique and let me have any references
to<br>
who did the modification and<br>
<br>
where it was published.<br>
<br>
Many Thanks,<br>
<br>
Tony Brandwood,<br>
Deputy Laboratory Superintendent,<br>
Department of Veterinary Pathology,<br>
University of Liverpool,<br>
P.O.Box 147,<br>
Liverpool,<br>
L69 3BX.<br>
U.K.<br>
<br>
Tel. No. 0151-794 4251<br>
Fax. No. 0151-794 4268<br>
e-mail afbrand@liv.ac.uk</font></blockquote><br>
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