Donna,

I dip my own P-L-L slides.  My supply source is Sigma Chemical Co., cat. #
P8920.  It comes concentrated (100 mls), and you dilute it 1:10.  Dip slides
for 5  minutes and air dry overnight in a dust-free location (or in warm
oven for an hour or so).  I tip my slides with label end down during the
drying process, in case any excess pools on the edge of the slide.  The
diluted P-L-L keeps fairly well for months, if refrigerated.

Jan Shivers
Univ. of Minn. Vet. Diag. Lab
St. Paul, MN

----- Original Message -----
From: "Donna Sitrin" <dsitrin@unipathllc.com>
To: <histonet@pathology.swmed.edu>
Sent: Wednesday, January 10, 2001 4:44 PM
Subject: Poly - L- lysine home brew


> Has anyone had extremely consistent results with making their own
> poly-l-lysine coated slides.  It has been years since I've done this and
the
> memory isn't what it used to be.
>
> If you have a good recipe with consistent results, please pass it along.
> This would be very helpful to me.  I'll appreciate all the help I can get.
>
> Thanks in advance,
> Donna Sitrin
> UniPath, LLc
> Denver
>
>
>