Re: Poly - L- lysine home brew

From:Jan Shivers <>


I dip my own P-L-L slides.  My supply source is Sigma Chemical Co., cat. #
P8920.  It comes concentrated (100 mls), and you dilute it 1:10.  Dip slides
for 5  minutes and air dry overnight in a dust-free location (or in warm
oven for an hour or so).  I tip my slides with label end down during the
drying process, in case any excess pools on the edge of the slide.  The
diluted P-L-L keeps fairly well for months, if refrigerated.

Jan Shivers
Univ. of Minn. Vet. Diag. Lab
St. Paul, MN

----- Original Message -----
From: "Donna Sitrin" <>
To: <>
Sent: Wednesday, January 10, 2001 4:44 PM
Subject: Poly - L- lysine home brew

> Has anyone had extremely consistent results with making their own
> poly-l-lysine coated slides.  It has been years since I've done this and
> memory isn't what it used to be.
> If you have a good recipe with consistent results, please pass it along.
> This would be very helpful to me.  I'll appreciate all the help I can get.
> Thanks in advance,
> Donna Sitrin
> UniPath, LLc
> Denver

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