Re: Poly - L- lysine home brew
|From:||Jan Shivers <firstname.lastname@example.org>|
I dip my own P-L-L slides. My supply source is Sigma Chemical Co., cat. #
P8920. It comes concentrated (100 mls), and you dilute it 1:10. Dip slides
for 5 minutes and air dry overnight in a dust-free location (or in warm
oven for an hour or so). I tip my slides with label end down during the
drying process, in case any excess pools on the edge of the slide. The
diluted P-L-L keeps fairly well for months, if refrigerated.
Univ. of Minn. Vet. Diag. Lab
St. Paul, MN
----- Original Message -----
From: "Donna Sitrin" <email@example.com>
Sent: Wednesday, January 10, 2001 4:44 PM
Subject: Poly - L- lysine home brew
> Has anyone had extremely consistent results with making their own
> poly-l-lysine coated slides. It has been years since I've done this and
> memory isn't what it used to be.
> If you have a good recipe with consistent results, please pass it along.
> This would be very helpful to me. I'll appreciate all the help I can get.
> Thanks in advance,
> Donna Sitrin
> UniPath, LLc
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