Re: Michel's transport medium (NOT fixative)

From:burch007@mc.duke.edu



This pertains to use of the Michel's Transport Medium. I guess we goofed on the
title. I always had a hard time getting the pH to stabilize. I recall having to
pH the solution over several days untill it read stable. Does anyone else have
this problem when making Michel's?

Jim Burchette

IMMUNOCYTOCHEMICAL DETECTION OF LYMPHOCYTE SURFACE ANTIGENS IN FIXED TISSUE
SECTIONS. MJ Borowitz, BP Croker, JL Burchette. Journal of Histochemistry and
Cytochemistry, Vol. 30, No. 2, pages 171-174, 1982.





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