Re: May-Grunwald

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From:Mary Latimer <> (by way of histonet)
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Please correct me but I always assumed that a virus was too small for
light microscopy.Inclusion bodies etc are the result of changes caused by
the virus rather than the virus itself.Mary

On Wed, 5 Jan 2000 wrote:

> Happy New Year everyone!
> I'm not very familiar with the use of Wright-Giemsa and May-Grunwald
>stains.  I've had limited experience in using these two stains.
> I understand that these two stains are two different staining
>formulations, but  I've been asked to find out if The May-Grunwald  stain
>will demonstrate viruses in any  form in paraffin sections.  If it does
>what is the expected result ( how will it be demonstrated) ?  Does it
>demonstrate Viral Inclusion bodies of only some viruses and not others?  I
>know a Wright Giemsa will demonstrate various parasites and bacteria on a
>blood smear, but do not know if it demonstrates viruses in Paraffin tissue
> The person I'm asking for is a customer that is specifically looking for
>Adenovirus.  I've suggested they find a vendor that sells the Adenovirus
>antibody, but they seem intent on using May-Grunwald.  If M-G works, would
>a regular Wright Giemsa work ?
> Your Knowledgeable input is ,as always, very much appreciated.
> Thanks
>     :)
> Mark Lewis

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