Re: May-Grunwald
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From: | Mary Latimer <ml4@st-andrews.ac.uk> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Please correct me but I always assumed that a virus was too small for
light microscopy.Inclusion bodies etc are the result of changes caused by
the virus rather than the virus itself.Mary
On Wed, 5 Jan 2000 mark.lewis@shandon.com wrote:
> Happy New Year everyone!
>
> I'm not very familiar with the use of Wright-Giemsa and May-Grunwald
>stains. I've had limited experience in using these two stains.
> I understand that these two stains are two different staining
>formulations, but I've been asked to find out if The May-Grunwald stain
>will demonstrate viruses in any form in paraffin sections. If it does
>what is the expected result ( how will it be demonstrated) ? Does it
>demonstrate Viral Inclusion bodies of only some viruses and not others? I
>know a Wright Giemsa will demonstrate various parasites and bacteria on a
>blood smear, but do not know if it demonstrates viruses in Paraffin tissue
>sections.
> The person I'm asking for is a customer that is specifically looking for
>Adenovirus. I've suggested they find a vendor that sells the Adenovirus
>antibody, but they seem intent on using May-Grunwald. If M-G works, would
>a regular Wright Giemsa work ?
>
> Your Knowledgeable input is ,as always, very much appreciated.
>
> Thanks
> :)
> Mark Lewis
>
>
>
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