Hi,
    Try saffranin red for about a minute then rinse and dehydrate quickly. It's
much faster than nuclear fast red (NFR) and it looks better too. By the way I
agree you probably need to wash longer for NFR.
Amos Brooks

Andrea Grantham wrote:

> Histonetters -
> I was wondering if there was another stain (other than Nuclear Fast Red)
> that could be used as a counterstain for the iron stains?
> My next question is - what can be done to prevent the white precipitate
> from adhering to the slides that have been in Nuclear Fast Red
> (Kernechtrot)Solution? I have had this problem with several batches of the
> stain - not just in this lab with these chemicals. I am sure that I am
> making up the solution correctly and just to be certain I had another tech
> make it up using the "recipe" from the green AFIP book (page 179). Still
> the same problem.
> Any advice/help would be greatly appreciated since this is driving me nuts!
> Thanks!
> Andi
> .....................................................................
> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :
> : Sr. Research Specialist       University of Arizona               :
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