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Andrea,<br>
If you are talking about a frosty looking film
on your slides after coverslipping, that is usually because the slides
were not rinsed well in distilled after nuclear fast red. I use
nuclear fast red almost every day without precip problems.<br>
<br>
Rena AS,HT(ASCP)<br>
Medical University of SC<br>
Charleston,SC<br>
<br>
<br>
<br>
At 08:43 AM 1/5/01 -0700, you wrote:<br>
<blockquote type=cite class=cite cite>Histonetters -<br>
I was wondering if there was another stain (other than Nuclear Fast Red)
that could be used as a counterstain for the iron stains?<br>
My next question is - what can be done to prevent the white precipitate
from adhering to the slides that have been in Nuclear Fast Red
(Kernechtrot)Solution? I have had this problem with several batches of
the stain - not just in this lab with these chemicals. I am sure that I
am making up the solution correctly and just to be certain I had another
tech make it up using the "recipe" from the green AFIP book
(page 179). Still the same problem.<br>
Any advice/help would be greatly appreciated since this is driving me
nuts!<br>
Thanks!<br>
Andi<br>
.....................................................................<br>
: Andrea Grantham, HT(ASCP) Dept. of Cell Biology
& Anatomy :<br>
: Sr. Research Specialist University
of
Arizona
:<br>
: (office: AHSC
4212) P.O. Box
245044
:<br>
: (voice: 520-626-4415)
Tucson, AZ 85724-5044
USA :<br>
: (FAX:
520-626-2097)
(email: algranth@u.arizona.edu)
:<br>
:...................................................................:<br>
<a href="http://www.cba.arizona.edu/histology-lab.html" eudora="autourl">http://www.cba.arizona.edu/histology-lab.html</a><br>
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