Re: Iron stain counterstain

From:Rena Fail <RFail@charleston.net>

<html> <font size=3><br> Andrea,<br>      If you are talking about a frosty looking film on your slides after coverslipping, that is usually because the slides were not rinsed well in distilled after nuclear fast red.  I use nuclear fast red almost every day without precip problems.<br> <br> Rena AS,HT(ASCP)<br> Medical University of SC<br> Charleston,SC<br> <br> <br> <br> At 08:43 AM 1/5/01 -0700, you wrote:<br> <blockquote type=cite class=cite cite>Histonetters -<br> I was wondering if there was another stain (other than Nuclear Fast Red) that could be used as a counterstain for the iron stains?<br> My next question is - what can be done to prevent the white precipitate from adhering to the slides that have been in Nuclear Fast Red (Kernechtrot)Solution? I have had this problem with several batches of the stain - not just in this lab with these chemicals. I am sure that I am making up the solution correctly and just to be certain I had another tech make it up using the "recipe" from the green AFIP book (page 179). Still the same problem.<br> Any advice/help would be greatly appreciated since this is driving me nuts!<br> Thanks!<br> Andi<br> .....................................................................<br> : Andrea Grantham, HT(ASCP)     Dept. of Cell Biology & Anatomy     :<br> : Sr. Research Specialist       University of Arizona               :<br> : (office:  AHSC 4212)          P.O. Box 245044                     :<br> : (voice:  520-626-4415)        Tucson, AZ  85724-5044    USA       :<br> : (FAX:  520-626-2097)          (email:  algranth@u.arizona.edu)       :<br> :...................................................................:<br>           <a href="http://www.cba.arizona.edu/histology-lab.html" eudora="autourl">http://www.cba.arizona.edu/histology-lab.html</a><br> <br> </font></blockquote><br> </html>
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