Just fix them in NBF, embed in paraffin, and do an H&E. Collagen gels
stain very lightly in an H&E and the fibroblasts will stain dark with the
hematoxylin. You shouldn't have any problem seeing/counting the
fibroblasts. Any problems, ask me about it; collagen matrices of one kind
or another account for the bulk of my lab's work.   jc

On Fri, 7 Jan 2000 02:06:41 -0800 (PST)
=?iso-8859-1?q?viola=20westerbarkey?= <vwesterbarkey@yahoo.com> writes:
>We are interested in invasion assays for fibroblasts
>in collagenic matrices. For this we want to make a
>collagen gel, seed fibroblasts onto the surface of the
>polymerized gels, incubate them, fix the gels  and
>look for the cells, which are invaded into the gel.
>Therefore I'm looking for a suitable method to detect
>the cells in the gels and to measure the migration
>distance of the fibroblasts.
>Is there anyone who has experiance with such an assay
>and can give us some hints doing this?
>__________________________________________________
>Do You Yahoo!?
>Talk to your friends online with Yahoo! Messenger.
>http://im.yahoo.com
>

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