Re: Has anybody used these antibodies?

From:Dana Settembre <settembr@UMDNJ.EDU>

On Thu, 4 Jan 2001 wrote:

> Hello fellow histonetters---
>                                   Just wondering if anyone has an opinion on the
> following 3 antibodies-MMP-9, EGFR, and Cox-2.  If someone has a prefered
> company, type of antigen retreival, whatever, I would love to hear from you.
> After I have worked these out I will post the results on the Histonet.  After
> all you can never have too many protocols!
> Simcerely,
> Christine Klein, BSMT(ASCP)
> MD Anderson Cancer Center, Houston, Tx.
Hello Christine Klein
I use Dako's EGFR code# M3563 with a placenta or skin as a positive
control.  I use prot. K for 5 minutes at RT and use the antibodya at a
1:20 dilution.

Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark,  New Jersey

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