Re: Has anybody used these antibodies?
From: | Dana Settembre <settembr@UMDNJ.EDU> |
On Thu, 4 Jan 2001 cklein@mail.mdanderson.org wrote:
> Hello fellow histonetters---
> Just wondering if anyone has an opinion on the
> following 3 antibodies-MMP-9, EGFR, and Cox-2. If someone has a prefered
> company, type of antigen retreival, whatever, I would love to hear from you.
> After I have worked these out I will post the results on the Histonet. After
> all you can never have too many protocols!
>
> Simcerely,
> Christine Klein, BSMT(ASCP)
> MD Anderson Cancer Center, Houston, Tx.
>
>
>
Hello Christine Klein
I use Dako's EGFR code# M3563 with a placenta or skin as a positive
control. I use prot. K for 5 minutes at RT and use the antibodya at a
1:20 dilution.
Dana Settembre
Immunohistochemistry Lab
Pathology Department
University Hospital
Newark, New Jersey
USA
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