Christine,

We have been using DAKO's EGFR.  We do not use an antigen retrieval, but
digest with ProteinaseK and use a dilution of 1:350.

Bonnie McMahill
Sacred Heart Medical Center/Pathology Associates Inc
Spokane, WA



------Original Message------
From: cklein@mail.mdanderson.org
To: histonet@pathology.swmed.edu
Sent: January 4, 2001 9:09:43 PM GMT
Subject: Re: Has anybody used these antibodies?


Hello fellow histonetters---
Just wondering if anyone has an opinion on the
following 3 antibodies-MMP-9, EGFR, and Cox-2.  If someone has a prefered
company, type of antigen retreival, whatever, I would love to hear from you.
After I have worked these out I will post the results on the Histonet. 
After
all you can never have too many protocols!

Simcerely,
Christine Klein, BSMT(ASCP)
MD Anderson Cancer Center, Houston, Tx.


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