Re: H & E-don't give up on Gill's

<< Previous Message | Next Message >>
From:"Sarah Christo" <> (by way of histonet)
Content-Type:text/plain; charset="us-ascii"

I disagree, I use my own preparation of Gill's #3 and use acid alcohol to
differentiate.  I have seen this technique routinely used at huge
university teaching hospitals (UTMB Galveston).   Bancroft's book states
that Gill #1 is regressive.  If #1 is regressive then surely #3 is, being a
stronger prep.   I'll stand up my Gills stain to any Harris for darkness.
Must depend on the hands it is in.  Sarah

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
Histology Laboratory
College Station, TX  77868-4458
phone (409) 845-3177
fax (409) 847-8981

>>> Shelley Sheridan <> 01/12 10:36 AM >>>

I would highly recommend using Harris instead of Gills.  You have more
control during differentiation because you can over-stain and then go back.
With Gills you are unable to do that.  Using Harris gave us a much darker
stain, which showed us more nuclear detail.

Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104

Do You Yahoo!?
Talk to your friends online with Yahoo! Messenger.

<< Previous Message | Next Message >>