Re: H & E-don't give up on Gill's

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From:"Sarah Christo" <schristo@cvm.tamu.edu> (by way of histonet)
To:histonet@histosearch.com
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I disagree, I use my own preparation of Gill's #3 and use acid alcohol to
differentiate.  I have seen this technique routinely used at huge
university teaching hospitals (UTMB Galveston).   Bancroft's book states
that Gill #1 is regressive.  If #1 is regressive then surely #3 is, being a
stronger prep.   I'll stand up my Gills stain to any Harris for darkness.
Must depend on the hands it is in.  Sarah

Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
Histology Laboratory
College Station, TX  77868-4458
schristo@cvm.tamu.edu
phone (409) 845-3177
fax (409) 847-8981

>>> Shelley Sheridan <sksherid@yahoo.com> 01/12 10:36 AM >>>

I would highly recommend using Harris instead of Gills.  You have more
control during differentiation because you can over-stain and then go back.
With Gills you are unable to do that.  Using Harris gave us a much darker
stain, which showed us more nuclear detail.




Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
Phone:(215)614-0051

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