Re: H & E-don't give up on Gill's
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From: | "Sarah Christo" <schristo@cvm.tamu.edu> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
I disagree, I use my own preparation of Gill's #3 and use acid alcohol to
differentiate. I have seen this technique routinely used at huge
university teaching hospitals (UTMB Galveston). Bancroft's book states
that Gill #1 is regressive. If #1 is regressive then surely #3 is, being a
stronger prep. I'll stand up my Gills stain to any Harris for darkness.
Must depend on the hands it is in. Sarah
Sarah Christo, HT (ASCP)
Texas A&M University
College of Veterinary Medicine
Dept. of Vet. Anatomy & Public Health
Histology Laboratory
College Station, TX 77868-4458
schristo@cvm.tamu.edu
phone (409) 845-3177
fax (409) 847-8981
>>> Shelley Sheridan <sksherid@yahoo.com> 01/12 10:36 AM >>>
I would highly recommend using Harris instead of Gills. You have more
control during differentiation because you can over-stain and then go back.
With Gills you are unable to do that. Using Harris gave us a much darker
stain, which showed us more nuclear detail.
Shelley K. Sheridan
Research Specialist
Center for Neurodegenerative Disease Research
University of Pennsylvania Medical School
Philadelphia, PA 19104
Phone:(215)614-0051
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