Re: Congo Red Stain problems
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From: | Ken Turner <ken.turner@stonebow.otago.ac.nz> (by way of histonet) |
To: | histonet@histosearch.com |
Reply-To: | |
Content-Type: | text/plain; charset="us-ascii" |
Debbie,
My understanding is that yellow birefringence is a result of a combination
of one or more of 3 factors; (i) Sections too thick
(ii) Orientation of amyloid fibrils
(iii) Quality of optics in use
Regards
Ken.
At 09:41 AM 1/13/00 -0600, you wrote:
> Hi everyone,
>I would like to ask if anyone knows why amyloid on Congo Red stain would
>birefringe as a orange-yellow color instead of apple green? We are using
>the same procedure that we have used for years. Our procedure is a Puchtler
>Modification. If anyone has any ideas, I would appreciate hearing from you.
>Thanks in advance.
>
>Debbie J. Siena, HT(ASCP)QIHC
>Histopathology Manager
>Baylor University Medical Center
>Dallas, TX 75246
>214-820-2465
>
>
Kenneth W Turner
Senior Teaching Fellow
Manager Histology Service Unit
Department of Pathology
Dunedin School of Medicine e-mail ken.turner@stonebow.otago.ac.nz
University of Otago Phone: (03) 479 7135 or (03) 479-7152
New Zealand Fax: (03) 479-7136
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