Richard,
In our laboratory, we use a polyclonal rabbit CD3 (Dako), with HIER (10 mn
in citrate buffer pressure cooker) since 6 years now and its works very well
(either after fixation with formalin or with Bouin's solution).

Emmanuel Carloz
Service d' Anatomie & cytologie pathologiques; Hôp. Sainte Anne; Toulon,
France.
zolrac@club-internet.fr

----- Original Message -----
From: Richard Cartun <Rcartun@harthosp.org>
To: <Histonet@pathology.swmed.edu>
Sent: Thursday, January 06, 2000 4:34 PM
Subject: CD3


> I am interested in hearing what Histoneters are using for the detection of
CD3+ cells in paraffin sections (pAb vs. mAb, digestion vs. HIER, etc.).
Thanks.
>
> R. Cartun
>